RGD多肽修饰的无机小牛骨粉对MC3T3-E1细胞黏附、增殖及分化的影响
Journal of Oral Science Research(2015)
Abstract
目的:研究小鼠前成骨细胞MC3T3-E1在RGD修饰的无机小牛骨粉上的黏附、增殖及分化.方法:浸泡法制备RGD/Bio-Oss复合材料.MTT法检测细胞1h、2h、3h黏附,扫描电镜观察细胞黏附形态,DNA浓度定量分析细胞1d、3d、5d、7d的增殖.碱性磷酸酶(ALP)试剂盒检测细胞7d、14d的分化.结果:1h、2h、3h细胞黏附结果Bio-Oss/RGD组明显高于Bio-Oss组及空白对照组;1d、3d、5d、7 d Bio-Oss/RGD组和Bio-Oss组的DNA浓度差别无统计学意义,但都低于空白对照组;细胞分化第14天时Bio-Oss/RGD组的结果明显高于Bio-Oss组与空白对照组.结论:RGD对细胞黏附及分化有一定的促进作用,但对细胞增殖无显著促进作用.
MoreKey words
RGD peptide,Bovine bone,MC3T3-E1,Adhesion
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