Wnt通路抑制剂XAV-939对牙髓干细胞成脂分化的影响
DOAJ (DOAJ: Directory of Open Access Journals)(2016)
Abstract
目的:通过体外实验探讨Wnt通路抑制剂XAV?939对牙髓干细胞增殖及成脂分化的影响。方法酶消化法培养牙髓干细胞,鉴定后采用CCK8试剂盒检测XAV?939对牙髓干细胞增殖的影响,油红O染色检测XAV?939对牙髓干细胞成脂分化的影响,qRT?PCR检测成脂分化过程中,XAV?939对Wnt通路相关基因糖原合成酶激酶3β(glycogen synthase kinase?3β, GSK3β)和β?catenin以及成脂分化相关基因过氧化物酶体增殖物激活受体γ2(peroxisome proliferator activated receptor?γ2, PPARγ2)和CCAAT增强子结合蛋白α(CCAAT/enhancer binding protein α, C/EBPα)的影响。结果牙髓干细胞成脂诱导14 d后,XAV?939上调GSK3β、PPARγ2和C/EBPα的表达,同时下调β?catenin的表达。结论 XAV?939可以通过抑制Wnt通路促进牙髓干细胞的成脂分化。
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Key words
adipogenic differentiation,stem cells
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