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ADAM28反义寡核苷酸对人牙龈成纤维细胞内ADAM28的基因表达的影响

Zheng ZHAO,Jie LI, Xiuna DING,Lei ZHOU,Degang SUN

Journal of Modern Stomatology（2019）

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Abstract
目的 探讨金属蛋白酶解离素28 (ADAM28)反义寡核苷酸对人牙龈成纤维细胞(HGFs)内ADAM28表达的抑制作用.方法 设计合成与ADAM28 mRNA特异序列互补的AS-ODN和正义对照S-ODN转染HGFs后,通过RT-PCR和Western blot检测细胞中ADAM28的表达差异.结果 ADAM28 AS-ODN转染HGFs后,在转录、翻译和蛋白水平HGFs内ADAM28的表达均明显减弱,统计学分析有显著性差异(P＜0.05).结论 ADAM28 AS-ODN可有效阻断HGFs内ADAM28的基因表达.
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