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抗辐射小鼠cDNA文库的全长cDNA功能鉴定

王芹,刘晓秋,徐畅,杜利清,孙志娟,王彦,刘强,宋力,李进

International Journal of Radiation Medicine and Nuclear Medicine（2013）

Cited 0|Views7
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Abstract
目的 鉴定IRM-2小鼠cDNA文库的全长cDNA功能.方法 以IRM-2小鼠的21条表达序列标签为引物进行PCR,从IRM-2小鼠全长cDNA文库中测定cDNA克隆的序列.观察小鼠胚胎成纤维细胞经6.5 Gy γ射线照射后全长cDNA的表达和全长cDNA转染对辐射敏感细胞共济失调性毛细血管扩张症(AT)5B1VA细胞生长的影响.结果 小鼠胚胎成纤维细胞经照射后,IRM-2小鼠4号、5号和2号cDNA表达水平均高于其亲本ICR小鼠和615小鼠.将全长cDNA转染AT5B1VA细胞,经照射后4号、5号和2号cDNA细胞存活率较高.结论 IRM-2小鼠4号、5号和2号全长cDNA具有较强的抗辐射功能.
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Oligonucleotide array sequence analysis,Gene library,IRM-2 mice
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