薄层色谱法鉴别参萸清眩膏
Military Medical Journal of South China(2017)
Abstract
目的 建立参萸清眩膏的薄层色谱法(thin layer chromatography,TLC)鉴别方法.方法 采用TLC法对山茱萸、肉苁蓉、枸杞子、熟地黄、菟丝子药材进行鉴别,展开剂分别为甲苯-乙酸乙酯-甲酸(20∶4∶0.5)、氯仿-甲醇(40∶1)、乙酸乙酯-氯仿-甲酸(3∶2∶1)、乙酸乙酯-甲醇-甲酸(16∶0.5∶2)、甲醇-冰醋酸-水(4∶1∶5),薄层板为硅胶G薄层板(10 cm×10 cm,厚度为0.20~0.25 mm).结果 山茱萸、肉苁蓉、枸杞子、熟地黄、菟丝子的TLC斑点清晰,分离良好,阴性无干扰.结论 薄层鉴别方法科学、简便、有效,可用于参萸清眩膏各组分的鉴别.
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Key words
Thin layer chromatography,Shenyuqingxuan Ointment,Identification,Cornus officinalis,Cistanche deserticolo
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