精制冠心片超高效液相色谱法特征图谱的研究
Chinese Journal of Hospital Pharmacy(2016)
Abstract
目的:建立精制冠心片的特征图谱.方法:采用UHPLC法.采用Dikma Endevaorsil C18色谱柱(2.1 mm×100 mm,1.8μm),柱温30℃,流速0.2 mL·min-1,流动相为乙腈-0.05%磷酸溶液,梯度洗脱,检测波长为270 nm.结果:建立了以丹酚酸B,丹参酮ⅡA为参照峰的精制冠心片的特征图谱,并指认了芍药苷、迷迭香酸、丹酚酸B、藁本内酯、隐丹参酮和丹参酮ⅡA 6个特征峰.结论:精制冠心片特征图谱的建立,可以更好地控制精制冠心片的质量.
MoreKey words
refined coronary tablets,specific chromatogram,UHPLC
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