高效液相色谱法测定蓼子七中没食子酸含量
China Pharmaceuticals(2017)
Abstract
目的 建立测定蓼子七中没食子酸含量的高效液相色谱法.方法 色谱柱采用InertSusTain C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(15:85:0.085),流速为1.0 mL/min,检测波长为270 nm,柱温为35℃.结果 蓼子七中没食子酸质量浓度在0.1415~0.8305μg/mL范围内与峰面积线性关系良好(R2=0.9999),平均回收率为98.35%,RSD为0.60%(n=6).结论 该方法简便、快速,稳定性好,可用于蓼子七中没食子酸含量的测定.
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Key words
HPLC,Antenoron Filiforme ( Thunb. ),gallic acid,content determination
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