高效液相色谱法测定复方西羚解毒丸中4种成分的含量
Herald of Medicine(2014)
Abstract
目的:采用高效液相梯度洗脱法建立复方西羚解毒丸中牛蒡子苷、牛蒡子苷元、连翘苷和连翘酯苷A含量测定方法。方法 HyPersil C18色谱柱(4.6 mm×200 mm,5μm);体积流量:0.9 mL·min-1;流动相A为甲醇_乙腈(2:1),流动相B为0.2%磷酸溶液梯度洗脱;检测波长280 nm。结果牛蒡子苷、牛蒡子苷元、连翘苷和连翘酯苷A分别在0.3026~6.0520μg(r=0.9994),0.0692~1.3840μg(r=0.9997),0.0142~0.2840μg(r=0.9991),0.0314~0.6280μg(r=0.9992)范围内进样量与峰面积呈良好的线性关系,平均加样回收率分别为98.25%,97.91%,97.67%,96.69%,RSD(n=6)分别为0.84%,1.39%,1.32%,0.57%。结论该方法简便、准确、灵敏、重复性好,可作为复方西羚解毒丸中牛蒡子苷、牛蒡子苷元、连翘苷和连翘酯苷A的含量控制方法。
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