HPLC法测定鱼肝油软膏中全反式维生素A醋酸酯的含量
Chinese Journal of Drug Application and Monitoring(2017)
Abstract
目的:建立HPLC法测定鱼肝油软膏中全反式维生素A醋酸酯的含量.方法:采用超声提取方法处理鱼肝油软膏,用HPLC法进行含量测定.色谱柱为Platisil ODS C18柱(250 mm×4.6 mm,5μm),流动相:甲醇-水(98:2),流速:1.0 mL·min-1,检测波长:328 nm,柱温:30℃.结果:维生素A在0.5879~7.3485 U·mL-1范围内线性关系良好(r=0.9999);样品平均回收率为99.78%(RSD=1.57%).结论:本方法简便、准确、重复性好,可用于鱼肝油软膏中全反式维生素A醋酸酯的含量测定.
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Key words
Cod liver oil ointment,Vitamin A acetate,HPLC,Content determination
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