N-乙酰半胱氨酸活性炭缓释微囊对大鼠肝纤维化中细胞内成纤信号分子表达的影响
Chinese Journal of Modern Applied Pharmacy(2016)
Abstract
目的 研究口服乙酰半胱氨酸活性炭缓释微囊(N-acetylcysteine activated carbon microcapsules,ACNAC)在大鼠肝纤维化治疗中肝细胞内成纤信号分子表达的影响.方法 以明胶包裹吸附乙酰半胱氨酸(N-acetylcysteine,NAC)的载药活性炭制备得到ACNAC,以四氯化碳(CC14)制备大鼠肝纤维化模型,ACNAC高、中、低剂量组(80,40,20 mg·kg-1·d-1,按照乙酰半胱氨酸计算)进行灌胃给药,NAC、水飞蓟宾为阳性对照药物.治疗8周后处死大鼠,通过大鼠肝功能指标、病理切片、免疫组织化学法评价ACNAC对胞内信号分子表达中的影响.结果 CC14大鼠肝纤维化模型制备成功,其大鼠肝组织病理切片HE染色和网状纤维染色表明其肝纤维化程度为Ⅲ~Ⅳ期,免疫组化显示胞内TGF-β1/Tβ-R1和Smad2/3大量表达.ACNAC、NAC和SM对肝纤维化大鼠血清ALT、AST及Tbil有显著的治疗作用,ACNAC高剂量组效果明显优于NAC和SM (P<0.05).ACNAC各治疗组大鼠肝组织病理切片表明肝纤维化好转,免疫组化显示胞内TGF-p1/Tβ-R1和Smad2/3表达减少,ACNAC高剂量组表达具显著性差异(P<0.05).结论 ACNAC对大鼠肝纤维化胞内成纤信号分子TGF-β1/Tβ-R1和Smad2/3有明显抑制作用,对抗大鼠肝纤维化的疗效明显,且较等剂量的NAC及对照药物水飞蓟宾有更显著的疗效.
MoreKey words
N-acetylcysteine ativated carbon sustained-release microcapsules (ACNAC),hepatic fibrosis,fibroblast signaling molecules
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