LC-MS/MS法测定人血浆中西格列汀浓度及其药物动力学研究
Chinese Journal of Clinical Pharmacology and Therapeutics(2016)
Abstract
目的:建立LC-MS/MS法测定人血浆中西格列汀的浓度并进行药动学研究.方法:人血浆样品用乙酸乙酯提取后,选用ZORBAX Eclipse XDB C18(2.1 mm×100 mm,3.5 μm)色谱柱,以乙腈-10 mmol/L醋酸铵溶液(80:20,含0.1%甲酸)为流动相,流速为0.30 mL/min,采用电喷雾离子化源,正离子方式,多反应监测(MRM)扫描方式进行监测,用于定量分析的离子反应分别为m/z 408.1→m/z 235.1(西格列汀)和m/z 303.2→n/z 152.9(内标橙皮素).结果:血浆中西格列汀的线性范围为0.20~1000 ng/mL(r=0.998 0),定量下限为0.20 ng/mL;方法回收率在93.2% ~ 105.5%;日内、日间RSD均小于15%;提取回收率81.6% ~ 86.5%,无明显基质效应.结论:该方法快速、灵敏、准确,专属性强,重复性好,适用于人血浆中西格列汀浓度的测定,可应用于西格列汀的血药浓度检测和药物动力学研究.
MoreKey words
sitagliptin,LC-MS/MS,plasma drug concentration,pharmacokinetics
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined