RP-HPLC 法测定盐酸加替沙星胶囊含量
Chinese Journal of Clinical Rational Drug Use(2014)
Abstract
目的:提高临床用药的质量控制,采用反向高效液相(RP-HPLC)法测定盐酸加替沙星胶囊含量。方法采用 Hypersil-ODSC18色谱柱,流动相:乙腈—水—十二烷基硫酸钠(500:500:3.5),检测波长:292nm;流速:1.0ml/ min。结果加替沙星在0.06~0.8mg/ ml 范围内线性关系良好,平均回收率为99.65%,RSD =0.78%(n =6)。结论 RP-HPLC 法具有简便,准确,测定结果可靠等优点。可作为盐酸加替沙星胶囊含量测定。
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