HPLC法测定金银花及降脂健心冲剂中绿原酸的含量
CHINESE JOURNAL OF PHARMACEUTICAL ANALYSIS(2003)
Abstract
目的:测定金银花及降脂健心冲剂中绿原酸的含量以控制产品质量.方法:采用Kromacil C18(4.6 mm×250 mm,5 μm)色谱柱;以乙腈-水-甲酸(40∶57∶3)为流动相,流速为1.3 mL*min-1,柱温为室温,检测波长为329 nm,以外标法测定了金银花药材及降脂健心冲剂中绿原酸的含量.结果:绿原酸在2.04~12.75 μg*mL-1的浓度范围内呈线性关系.绿原酸在制剂中的平均回收率(n=5)为100.2%(RSD=1.9%).结论:该方法准确、重现性好、简便、易行,可用于质量控制.
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