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不同产地金樱子根UPLC指纹图谱的研究

Yulu Li,Guoxu Ma,Jingquan Yuan,Wen Zhang,Jieming Zou,Xiaoqing Zhong,Yanlin Zhou,Zuolin Su,Nailiang Zhu,Xudong Xu,Wei Yi

China Pharmacist（2017）

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Abstract
目的:利用超高效液相色谱法建立金樱子根指纹图谱分析方法.方法:采用ACQUITY UPLC?Phenyl色谱柱(2.1 mm×100 mm,1.7μm),甲醇-水为流动相梯度洗脱,流速为0.2 ml·min-1,检测波长为210 nm,柱温为30℃,进样量为3μl.结果:标定了15个共有峰,得出对照指纹图谱,12批金樱子根药材中7批药材相似度>0.90,说明不同产地金樱子根药材指纹图谱既有共性又存在一定差异.结论:该指纹图谱分析方法操作简便、重复性好,可为科学地控制金樱子根的质量,合理地开发其药用资源提供依据.
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Roots of Rosa laevigata Michx,UPLC,Fingerprint,Mutual mode
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