HPLC法测定不同产地垂盆草药材中木犀草苷的含量
wf(2016)
Abstract
目的::建立反相高效液相色谱法测定垂盆草药材中木犀草苷的含量。方法:采用Waters SymmetryShield C18(250 mm ×4.6 mm,5μm)色谱柱;以四氢呋喃-甲醇-水-磷酸(9∶17∶74∶0.25)为流动相,流速为1.0 ml·min-1,检测波长为350 nm,柱温为35℃,进样量为10μl。结果:木犀草苷在5.2~208.0μg·ml-1(r=0.9999)范围内呈现良好的线性关系;平均回收率为99.12%,RSD=0.94%(n=6)。结论:该方法简便、灵敏、准确、专属性强,重复性好,可作为垂盆草药材中木犀草苷含量测定的方法。
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Key words
HPLC,Sedum Sarmentosum Bunge,Luteolin-7-O-glucoside
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