UHPLC-DAD法同时测定精制冠心片中6种成分含量

目的：：建立同时测定精制冠心片中芍药苷、迷迭香酸、丹酚酸B、藁本内酯、隐丹参酮和丹参酮ⅡA含量的UHPLC-DAD检测方法。方法：采用Dikma Endevaorsil C18色谱柱(2.1 mm ×100 mm,1.8μm),柱温：30℃,流速：0.3 ml·min-1,流动相为乙腈-0.05%磷酸溶液,梯度洗脱,检测波长为230,270,288,321 nm。结果：芍药苷、迷迭香酸、丹酚酸B、藁本内酯、隐丹参酮、丹参酮ⅡA分别在0.0010~0.0102μg(r =0.9998),0.0057~0.0569μg(r =1.0000),0.0053~0.0527μg(r =1.0000),0.0021~0.0206μg(r=1.0000),0.0011~0.0112μg(r=1.0000),0.0014~0.0144μg(r=0.9998)范围内呈良好的线性关系,平均加样回收率分别为98.78%(RSD=0.50%),97.99%(RSD=0.76%),98.44%(RSD=0.85%),99.12%(RSD=0.66%),98.82%(RSD=0.81%),97.80%(RSD=0.80%)。结论：该方法简便、快速、准确,可用于精制冠心片的质量控制。