HPLC法测定调经助孕颗粒中马钱苷的含量
China Pharmacist(2014)
Abstract
目的::建立调经助孕颗粒中马钱苷的含量测定方法。方法:采用高效液相色谱法,色谱柱为SinoChrom ODS-BP (250 mm ×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(14∶86),检测波长为240 nm,柱温30℃。结果:马钱苷进样量在50.40~1008.00 ng范围内与峰面积有良好的线性关系(r=0.9999),平均加样回收率为99.33%,RSD为1.41%(n=6)。结论:该方法快速、准确,操作简单,专属性强,可作为调经助孕颗粒中马钱苷的含量测定方法。
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Key words
Tiaojing Zhuyun granules,Loganin,HPLC
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