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添加甘油对北里链霉菌ZY150504发酵生产吉他霉素的影响

Qian-li Zheng,Shu-hong Gao, Jing Zhang, Jun-lei Zhao,Zhen Zhao, Pan Gao

Chinese Journal of Antibiotics(2016)

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Abstract
本文研究了添加甘油对北里链霉菌ZY150504发酵吉他霉素效价和A5组分的影响.考察了甘油的添加浓度和添加时间对发酵液效价的影响,发现通过添加甘油,提高发酵培养基中的起始甘油浓度,有效地提高了吉他霉素的效价.发酵结束时发酵液的效价为10847U/mL,比对照提高了47%,比文献报道效价提高了27%;吉他霉素A5组分含量提高17%;而A4组分含量降低了36%.进一步研究发酵过程发现添加甘油之后,发酵过程中条件组的脂肪酶活力较高,油脂利用较快,丙酸含量增加,乙酸浓度较低,促进了吉他霉素的生物合成,提高了A5组分的含量.
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Key words
Glycerol,Kitasamycin,Component,Streptomyces kitasatoensis
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