阴离子交换固相萃取-HPLC快速测定中药材中合成色素的方法研究
Journal of North Pharmacy(2016)
Abstract
目的:针对中药材染色现象,建立快速测定中药材中亮蓝、柠檬黄、日落黄、赤藓红、苋菜红、诱惑红等6种合成色素的HPLC检测方法。方法:样品用甲醇-0.1%甲酸溶液提取后,再经阴离子固相萃取去除大部分基质干扰,采用Agilent C18色谱柱(4.6mm×250mm,5μm),以甲醇-20mmol/L乙酸铵为流动相,梯度洗脱,以二极管阵列检测器测定。结果:在山茱萸、黄柏两种基质中,这6种色素在0.1μg/L至100μg/L范围内线性关系良好。检测限为0.16~1.04mg/kg,回收率为80.5%~102.9%,相对标准偏差为1.6%~4.8%。结论:本方法操作简单,结果准确可靠,重现性好,适用于中药材中多种人工色素的快速检测。
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Key words
Nion exchange SPE,HPLC,Medicinal Chinese herb,Synthetic pigments
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