HPLC测定清热解毒口服液中4个成分的含量
West China Journal of Pharmaceutical Sciences(2016)
Abstract
目的 采用HPLC法测定清热解毒口服液中黄芩苷、绿原酸、连翘苷、连翘酯苷A的含量.方法 采用Zorbax EclipseXDB C18色谱柱(150 mm ×4.6 mm,5μm),柱温30℃,流动相为乙腈-0.3%磷酸溶液,梯度洗脱,流速1.0 mL· min-1,检测波长324 nm(绿原酸、连翘酯苷A)、276 nm(黄芩苷、连翘苷).结果 黄芩苷、绿原酸、连翘苷、连翘酯苷A的线性范围分别为0.182 ~0.912、0.061~0.304、0.037 ~0.183、0.013 ~0.065 μg,加样回收率分别为98.82%、96.94%、105.6%、95.74%(n=6).结论 所用方法操作简单,为全面控制清热解毒口服液的质量提供了一种可靠的检测方法.
MoreKey words
Qingrejiedu oral liquid,Baicalin,Chlorogenic acid,Forsythin,Forsythoside A,HPLC,Dual-wavelength,Determination
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