胆固醇软膏的比色法测定

CHINESE JOURNAL OF PHARMACEUTICALS(2005)

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Abstract
建立了比色法测定胆固醇软膏的含量.用甲醇-氯仿(1:2)提取,邻苯二甲醛-冰乙酸-浓硫酸显色,于555nm处比色测定.结果显示,胆固醇在120~200μg/ml浓度范围内线性关系良好(r=0.9999),回收率大于98%(RSD<1.5%).
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