Cellular chemokine CCL18 increases matrix metalloproteinases expression and promotes ovarian cancer cell invasion and metastasis in vivo

Objective:To study the effect of cell chemokine (C-C motif) ligand 18 (CCL18) on ovarian cancer proliferation and metastasis.Methods:This study first analyzed the effects of overexpression of CCL18 on the growth in a subcutaneous ovarian cancer xenografts mouse model.Real-time quantitative PCR was used to detect the expression of matrix metalloproteinases (MMPs) in xenografts tissues.Then,an ovarian cancer orthotropic xenografts model was used to access the effect of CCL18 on ovarian cancer metastasis.Results:Over expressing CCL18 in SKOV3 cells did not significantly promote the tumor growth of subcutaneous xenografts.But the mRNA levels of MMP1,MMP7,MMP11 and MMP15 were significantly inncreased (P ＜ 0.05).The mRNA level of MMP12 was not changed (P ＞0.05).In orthotopic xenografts ovarian cancer mouse model,metastasis appeared in more organs in CCL 18 overexpressed SKOV3 cells than GFP/SKOV3 cells.Conclusion:CCL18 increased the expresston of MMPs in ovarian cancer cells and promoted metastasis of ovarian cancer cells in vivo.