高效液相色谱法测定四味光明盐汤散中胡椒碱的含量
Journal of Inner Mongolia Medical University(2014)
Abstract
目的:建立四味光明盐汤散中胡椒碱的含量测定方法。方法:高效液相色谱法。 Phenomen C18色谱柱(250 mm×4.6 mm,5μm);甲醇-水(77:23)为流动相;流速为1.0 mL/min;检测波长为343nm;柱温30℃。结果:胡椒碱在50.80 ng~508.0 ng范围内与峰面积积分值呈良好的线性关系,回归方程为y=664890 x+53019,r=1.0000。低、中、高浓度平均加样回收率分别为99.31%、102.4%、102.4%, RSD%分别为0.84%、0.74%、0.45%。结论:该方法准确、精密、重复性好,可作为四味光明盐汤散中胡椒碱的含量测定方法。
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