响应面法优化黑种草皂苷A双水相提取工艺研究

Journal of Xinjiang Medical University(2019)

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Abstract
目的 优化黑种草皂苷A双水相提取工艺.方法 采用响应面法,以黑种草皂苷A提取率为指标,考察料液比、硫酸铵用量、乙醇浓度和超声时间对黑种草皂苷A提取的影响.结果 黑种草皂苷A最佳提取工艺为:料液比1:40,硫酸铵浓度0.35 g/mL,乙醇浓度47%,超声时间30 min,在此条件下黑种草皂苷A的提取率为3.15%.结论 该工艺简单、可靠,适用于黑种草皂苷A的提取.
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