人B7-2基因修饰的食管癌细胞瘤苗抗肿瘤的免疫效应

Guoqiang ZHAO,Liming TANG,Jing LU,Jun ZHAO,Qian SONG,Ziyou CUI,Hongyan Yang,Jimin Zhao,Youtian Huang,Ziming DONG

JOURNAL OF ZHENGZHOU UNIVERISTY(MEDICAL SCIENCES)（2005）

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Abstract

目的:研究人B7-2基因修饰的食管癌细胞作为瘤苗的抗肿瘤作用.方法:将真核荧光表达载体pEGFP-C3-B7-2,通过脂质体转染技术转染人食管癌细胞株EC9706.外周血来源的树突状细胞(DC)负载肿瘤抗原后,与自体T淋巴细胞共培养3 d,获得细胞毒性T淋巴细胞(CTL).用四甲基偶氮唑蓝(MTT)法检测CTL对转染和未转染pEGFP-C3-B7-2的人食管癌细胞EC9706的杀伤活性.结果:CTL对转染pEGFP-C3-B7-2的肿瘤细胞的杀伤活性大于转染pEGFP-C3和未转染细胞的杀伤活性(P＜0.05).结论:人B7-2基因修饰的EC9706肿瘤细胞瘤苗,可诱导出明显的抗EC9706细胞的免疫效应.

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Key words

Cancer Immunoediting,Cancer Metabolism,Tumor Targeting

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