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核酸疫苗pcDNA3-cC1小鼠体内表达的血清学分析

章亚南,何晓文,王芳,姜磊,任丁,李德安,孙树汉

ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY（2005）

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Abstract
抗原cC1 是从猪囊尾蚴cDNA 文库中以囊虫病患者、病猪血清为探针筛选出的抗原蛋白, 经免疫学检测证实为具有较高特异性的诊断用抗原[ 1] .在前期的研究[ 1] 中,我们将全长cC1 cDNA 插入真核表达质粒载体pcDNA 3, 构建了重组质粒pcDNA3-cC1.该DNA疫苗免疫仔猪后,能有效诱导仔猪的免疫保护效应.本研究选择该DNA疫苗为分析对象,旨在建立一种早期预测DNA疫苗免疫效果的方法.
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