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圆柏花粉浸液40 kD蛋白组分的鉴定

Wenjing Li,Nan Huang,Guanghui Liu

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong（2014）

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Abstract
目的 对圆柏花粉浸液40 kD条带的蛋白组分进行初步鉴定,明确其一级结构.方法 将圆柏花粉浸液使用SDS-PAGE初步分离后,取40 kD条带进行质谱分析.结果 40 kD条带中获得肽段分别与Jun v 1,Cup a 1,Jun a 2及其他植物蛋白序列一致.结论 40 kD条带可能包含多种成分,包括柏科花粉1组2组变应原等.
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Key words
Juniperus chinensis pollen extract,40 kD protein band,mass spectrometry
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