HPLC测定复方甘草胶囊浸膏中苦参碱、氧化苦参碱含量
Journal of Tianjin Medical University(2017)
Abstract
目的:建立HPLC同时测定复方甘草胶囊中间体浸膏中苦参碱和氧化苦参碱的含量,为制备胶囊提供依据.方法:色谱柱为Kromasil-NH2柱(250 mm×4.6 mm,5μm),流动相为乙腈-无水乙醇-3%磷酸(80:10:10,V/V/V),流速1.0 mL/min,检测波长220 nm,柱温30℃.结果:苦参碱在5.12~30.72 μg/mL(r=0.999 5),氧化苦参碱在5.16~30.96 μg/mL(r=0.999 5)浓度范围内与峰面积线性关系良好,苦参碱平均回收率99.63%,RSD为0.62%(n=6),氧化苦参碱平均回收率99.81%,RSD为0.72%(n=6).结论:该方法准确,稳定性好,重复性高,可用于复方甘草胶囊中间体浸膏的质量控制,为胶囊制备提供依据.
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