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DTT诱导P38核转位与食管癌Eca109细胞凋亡的研究

冯若,刘国红,翟文龙,金辉,张钦宪

JOURNAL OF HENAN MEDICAL COLLEGE FOR STAFF AND WORKERS（2008）

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Abstract
目的 探讨P38的核转位与DTT诱导的食管癌细胞凋亡的关系.方法 采用DTT处理食管癌Eca109细胞.未加药组作为对照.流式细胞仪技术检测细胞凋亡率;免疫组化方法检测P38的磷酸化水平及磷酸化P38的核转位情况.结果 与对照组相比,DTT处理组细胞凋亡率明显升高.免疫组化结果显示:处理组P38的磷酸化水平明显高于对照组(P<0.01),且处理组P-P38大部分定位于核内.结论 P-P38核转位参与了DTT诱导的食管癌Eca109细胞凋亡.
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Key words
dtt induced nuclear translocation,apoptosis,nuclear translocation,p38
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