高效液相色谱法测定消炎止痢灵片中甲氧苄啶的含量
wf(2016)
Abstract
目的:建立测定消炎止痢灵片中甲氧苄啶含量的高效液相色谱法。方法采用高效液相色谱法,色谱柱为 Phenomenex Luna C18(5μm,4.6 mm ×250 mm),流动相为乙腈-0.5%磷酸溶液(含1 mmol·L -1醋酸铵)(33∶67),检测波长为270 nm。结果甲氧苄啶在0.1002~3.002μg 范围内与峰面积值呈良好的线性关系(相关系数为0.9999);平均回收率(n =6)为99.59%,RSD 为0.22%。结论该方法简单,准确性高,精密度好,适合消炎止痢灵片中甲氧苄啶的含量测定。
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