PGEX-5X-I-EGFR-COM112载体构建及蛋白纯化表达
Medical Information（2015）
Abstract
目的：为获得PGEX-5X-I载体与EGFR-COM112的融合蛋白。方法将EGFR-COM112基因克隆入已构建好的载体PGEX-5X-I中,转化大肠杆菌Top10。该菌株经IPTG诱导后,高效表达出融合蛋白PGEX-5X-I-EGFR-COM112,经PCR和测序鉴定正确的重组质粒。经PBS稀释复性、Ni离子亲和层析柱纯化,获得目的蛋白。结果 SDS-PAGE分析表明,在38 kD左右有一条明显的特异性蛋白条带。结论成功地制备了EGFR-COM112的载体并纯化其蛋白表达。
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