LDL与Raw264.7细胞中肾素-血管紧张素系统表达

Nan Liu, Cheng Zhang,Shujia Wu,Junzhu Wu

Yiyao Qianyan（2019）

Cited 0|Views8

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Abstract

目的:探究巨噬细胞R a w264.7氧化低密度脂蛋白(L D L)过程中肾素-血管紧张素系统(R A S)的变化.方法:培养RAW264.7细胞,LDL刺激后,用ACE活性测定试剂盒检测细胞内ACE活性的变化,用AngⅡ试剂盒测定AngⅡ的表达;Real-Time PCR检测血管紧张素转移酶(ACE)mRNA的表达;利用Real-Time PCR及Western Blot检测血管紧张素Ⅱ2型受体(AGTR2、AT2R)的表达.结果:LDL刺激Raw264.7细胞后,细胞内ACE的活性、AngⅡ的表达、ACE mRNA水平、AT2R mRNA和蛋白质的表达都升高(P<0.05).结论:LDL激活了肾素-血管紧张素系统.

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