Survivin靶向siRNA重组表达载体的构建与鉴定
World Health Digest(2013)
Abstract
目的构建Survivin靶向siRNA重组表达载体。方法根据GeneBank数据库提供的Survivin基因所有变异体的共同核苷酸序列,选择设计一对带发夹结构的核苷酸序列,克隆到空载体pSIREN-DNR-DsRed-Express中,转化DH5а菌株,提取质粒进行酶切鉴定和测序分析以及菌落PCR。结果经质粒PCR、菌落PC及测序证实Survivin靶向siRNA重组表达载体结果与设计完全一致。结论 Survivin靶向siRNA重组表达载体构建成功。
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