CD44重组表达抑制鼻咽癌6-10B细胞增殖
Acta Medicinae Sinica（2013）
Abstract
目的:探索CD44v10剪接变异体对鼻咽癌细胞增殖的生物学作用.方法:将CD44v10基因编码序列重组,构建pIRESne03-CD44v10重组质粒表达载体,重组表达载体扩增后采用脂质体转入鼻咽癌6-10B细胞系,G418筛选稳定转染细胞株;RT-PCR和蛋白印迹鉴定CD44v10重组表达6-10B细胞株;MTT法分析细胞增殖.结果:获得了CD44v10稳定重组表达的6-10B细胞株;CD44v10表达抑制6-10B细胞增殖.结论:CD44v10表达对鼻咽癌细胞的恶性增殖可能具有抑制作用.
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Key words
recombinant cd44,nasopharyngeal carcinoma
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