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HPLC测定参柏舒心颗粒中盐酸黄柏碱含量

Wei Wang,Chunxia Xiao,Guangli Ai,Xufeng Pu

Asia-Pacific Traditional Medicine（2016）

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Abstract
目的:建立参柏舒心颗粒中黄柏碱含量测定的方法.方法:采用Diamonsil-C18(5μm,4.6mm×250mm)色谱柱,以乙睛—0.1％磷酸溶液(36:64)(每100mL加十二烷基磺酸钠0.1g)为流动相,流速:1mL·min-1,检测波长284nm,柱温35℃.结果:盐酸黄柏碱在0.0586～1.1730 μg范围内峰面积与浓度呈良好的线性关系,平均加样回收率为100.37％,RSD=1.57％.结论:该方法简便准确,专属性强,可用于参柏舒心颗粒质量控制的方法.
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