蚕沙分离的溶磷解钾菌株OP6发酵配方
AGRICULTURAL ENGINEERING(2019)
Abstract
发酵是功能微生物菌株实用化的重要环节.以蚕沙中分离的耐高温、具溶磷解钾功能的巨大芽孢杆菌OP6菌株为目标菌株,采用单因素试验研究了其培养基中的碳源、氮源和金属离子等因子对OP6繁殖数量的影响,筛选获得最优发酵配方:玉米淀粉3%,豆粕粉3%,酵母膏0.9%,NaC1 2.5%,CaCO32.5%,MgSO40.4%,KH2PO40.3%,MnSO40.075%,pH值7.2 ~7.5.并对该配方进行了三角瓶和30 L发酵罐验证.
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