荠菜总基因组DNA提取方法的研究
Agricultural Development and Equipments(2016)
Abstract
采用改良CTAB法、CTAB法、SDS法和高盐低pH法分别对荠菜叶片进行基因组DNA的提取,比较4种方法提取DNA的浓度、纯度和完整性.结果表明,改良CTAB法获得的DNA浓度最高,为3105.85μg/ml,并且纯度高、完整性好、过程简单,确定荠菜基因组DNA的最佳提取方法为改良CTAB法.
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