银杏无菌苗培养方法的优化
Seed（2018）
Abstract
分别以银杏种核和种胚为试材进行无菌苗的培养,对接种材料、培养基类型和银杏胚乳汁浓度进行探讨,探寻适合银杏无菌苗生长的最佳培养基配方.结果表明:以种胚为接种材料培养成的无菌苗生长情况较好,发芽率较高;相比于MS培养基,WPM培养基更适合做基本培养基进行银杏无菌苗的培养;在添加3％胚乳汁的WPM培养基条件下,无菌苗的生长情况最好,发芽率及生根率都保持在90％以上.
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