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淡紫拟青霉诱变菌株的RAPD分析

Yan-xin ZHANG,Kai-qi LIU,Zhen-yuan XIA,Tian-fei LI

CHINESE JOURNAL OF BIOLOGICAL CONTROL（2005）

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Abstract
选用6个随机引物对淡紫拟青霉IPC菌株及其15株突变菌株的全基因组DNA进行随机多态性扩增,共扩增出114条250～2500bp的DNA片段,多态检测率为83.3%.利用UPGMA方法对扩增的DNA片段聚类分析,结果表明:供试菌株具有丰富的遗传多样性,应用RAPD技术可以快速准确地鉴定菌株是否突变及突变程度;参照突变菌株表型特性上的改变,该方法可以作为确定其遗传性上是否发生分化的依据.
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paecilomyces,rapd analysis
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