柠檬树苗中截获的柑橘半穿刺线虫形态和分子鉴定

Plant Quarantine(2019)

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Abstract
上海口岸从旅客携带进境的柠檬树苗中分离到1种半穿刺属线虫的2龄幼虫及雄虫,通过形态特征和分子特征分析,鉴定为柑橘半穿刺线虫(Tylenchulus semipenetrans Cobb,1913).该线虫的主要形态鉴别特征为:雄虫虫体细,头部光滑、锥圆,口针和食道退化,排泄孔位于虫体中后部,单精巢、前伸,交合刺细、弓状,无交合伞,尾长圆锥形、端圆.2龄幼虫无直肠和肛门,排泄孔位于虫体中后部,尾部渐细、钝圆.这是全国口岸系统首次截获该线虫.
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