稻瘟菌MoHYR1蛋白的原核表达与纯化

The gene MoHYR1 is important for full virulence and H2O2tolerance in the rice blast fungus Magna porthe oryzae.MoHYR1 was obtained by PCR with the template of M.oryzae cDNA and cloned into a prokaryotic expression vector pHAT2.The protein MoHYR1 was successfully expressed in strain Escherichia coli BL21 (DE3) with an estimated molecular mass of 20 kDa.The protein was then purified by affinity chromatography and gel filtration.Finally,high purity of MoHYR1 was obtained,which contributes to further study on its structure and function.