延边烟区烟草菌核病病原菌鉴定及抗性种质筛选

Chong GAO,Guohe WU,Bailin LI,Chengrong AN,Genong GAO,Baohui LU,Yuliang GAO,Huimin TIAN,Chengrui ZHENG,Changfan CUI

Chinese Tobacco Science（2018）

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Abstract

为防控延边烟区烟草菌核病提供基础资料和理论依据,开展了病原菌鉴定及抗性种质材料筛选研究.将收集的病原菌核采用常规方法进行分离、纯化,经致病性测定、形态学特征观察、rDNA-ITS测序比对及基于rDNA-ITS序列的系统发育分析,鉴定病原菌种类;并采用离体叶片法、活体叶片法对61份种质材料进行抗性筛选.结果表明:(1)经形态学、分子生物学方法鉴定该病原菌为核盘菌[Sclerotinia sclerotiorum(Lib.)de Bary].(2)两种方法筛选出31份高感材料、6份中感材料、15份抗病材料,鉴定结果一致率为85.25%,未筛选到免疫和高抗种质材料.延边烟区烟草菌核病病原菌为核盘菌,离体叶片法、活体叶片法可用于苗期烟草种质材料对菌核病抗性筛选.

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