微波炉烘干棉花叶片用于DNA提取的效果分析
China Cotton(2020)
Abstract
采集棉花未展真叶、平展嫩叶和平展成熟叶用微波炉中火烘干后提取DNA,简单序列重复(Simple sequence repeat,SSR)引物扩增后的图谱显示未展真叶的DNA扩增效果不好.进一步开展了用平展子叶和全展真叶烘干的试验,发现平展子叶和全展真叶在微波炉低火、中火和高火条件下烘干后均能获得质量较好的DNA,能够满足后续分子试验的要求.烘干叶片密闭存放1周后仍能获得较好的DNA.烘干叶片提取的DNA OD260/OD280比值偏高,但不影响后续的SSR分子鉴定试验.
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