参薯组织培养的多因子正交试验研究

Zhencheng MENG,Cui SU,Xi YANG,Dexin KONG

Chinese Journal of Tropical Agriculture（2018）

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Abstract

以参薯茎段为研究材料,使用0.1％HgCl2进行不同消毒时间的消毒处理,并应用正交试验设计对参薯无菌苗的诱导、生根培养进行研究.结果表明:参薯茎段以0.1％HgCl2消毒7 min效果较好,污染率低,存活率及发芽率较高;最佳诱导培养基为:MS+6-BA 0.5 mg/L+GA30.1 mg/L+AC 1.0 g/L+蔗糖30 g/L;最佳生根培养基为:1/2 MS+NAA 0.5 mg/L+IBA 0.5 mg/L+蔗糖25 g/L+AC 0.5 g/L;添加0.5～1 g/L的AC能有效减小参薯组培苗的褐化.

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