鄂柿1号离体叶片秋水仙素诱导和再生植株倍性鉴定
JOURNAL OF FRUIT SCIENCE(2005)
Abstract
以鄂柿1号甜柿离体新梢叶片为外植体,研究了秋水仙素浓度及处理时间对诱导染色体倍性变异的影响.结果表明,0.5%秋水仙素处理4 d左右,可诱导获得十二倍体再生植株,低浓度的秋水仙素不能产生加倍效应,高浓度会导致叶片死亡或严重抑制愈伤组织再生能力;与对照比较,再生变异植株叶片气孔孔径增大、密度降低,PA倍性分析叶片DNA含量为对照的2倍.因此,试验获得的倍性变异株初步确认为12倍体.
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Key words
persimmon,regenerated plants,colchicine,vitro,eshi
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