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沙冬青AmCSDP基因特性及转基因烟草的抗寒性分析

WANG Honglei,WANG Yanping,YU Tingqiao,LIU Shengli,CHEN Yuzhen,LU Cunfu

Acta Horticulturae Sinica（2017）

Cited 3|Views2
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Abstract
冷休克结构域蛋白(CSPs)是含有不同数量冷休克结构域(cold shock domain,CSD)的低温响应蛋白,在低温胁迫中发挥重要作用.以沙冬青(Ammopiptanthus mongolicus)为材料,克隆得到了冷休克结构域蛋白基因AmCSDP.生物信息学分析显示AmCSDP(GenBank登录号:KX756575)全长540bp,编码180个氨基酸,二级结构由8.9％的α-螺旋、47.8％的β-折叠和43.3％的无规则卷曲组成,其N端含有冷休克结构域.进化分析表明沙冬青与花生同源性最高.构建了AmCSDP真核表达载体Pcambia2300-35 S-AmCSDP-OCS,经农杆菌介导转入本氏烟中.对转基因T1代和野生型烟草进行4℃低温胁迫处理,结果证实转基因本氏烟比野生型的耐冷能力强.
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Key words
cold tolerance,cold shock domain protein gene AmCSDP,Ammopiptanthus mongolicus,transgene
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