芋ADP-葡萄糖焦磷酸化酶大亚基cDNA片段的克隆

ACTA HOUTICULTURAE SINICA(2001)

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Abstract
利用ADP-葡萄糖焦磷酸化酶的保守序列设计引物,采用RT-PCR方法,从白魔芋球茎组织的总RNA中扩增出目标cDNA片段,并克隆到pUC18载体中。序列分析后确认该片段属于一新的ADP-葡萄糖焦磷酸化酶大亚基基因。
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