西番莲脱分化再生体系研究

Zhen LU,Chengmei HUANG,Yuanwen WEI,Zhinian DENG,Huiqing CAO,Kaichao WU,Haibin LUO,Shengli JIANG,Lin XU

China Fruits（2019）

Cited 1|Views11

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Abstract

以大果黄果西番莲为材料,通过无菌播种获得无菌苗,采用无菌苗的子叶及下胚轴为外植体,对诱导分化不定芽、不定芽继代增殖、生根诱导等方面进行研究,为西番莲组织培养和遗传转化研究提供基础.结果表明:以未完全展开的子叶为外植体,诱导不定芽分化的效果最好,最适宜的分化培养基为MS+ 2.0 mg/L 6-BA+ 0.1 mg/L IAA,其诱导分化率可达100.0％;最适宜的增殖培养基为MS+ 1.0 mg/L 6-BA+ 0.2 mg/L NAA,其芽苗增殖系数最高,为3.19,且芽苗长势好;最适宜的生根培养基为1/2MS+ 0.5 mg/L NAA+ 0.2 mg/L IBA,其生根率达100.0％,根粗壮、长、多,且幼苗长势好.

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