农杆菌介导法将小麦γ-硫堇蛋白基因转入厚皮甜瓜
CHINA VEGETABLES（2008）
Abstract
以厚皮甜瓜鲁厚甜2号为材料,在绿色叶肉组织特异表达启动子PNZIP驱动下,利用农杆菌介导的遗传转化系统,将小麦抗真菌γ-硫堇蛋白(γ-Thionin)基因(THI)导入甜瓜,获得卡那霉素抗性植株.转化植株经PCR和Northern blot检测分析,证实γ-硫堇蛋白基因已经转入甜瓜基因组中并在甜瓜叶片组织中表达.
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