大苞鞘石斛原球茎悬浮培养研究
ZHONGGUO YUANYI WENZHAI(2016)
Abstract
以大苞鞘石斛类原球茎为材料,通过悬浮培养,研究基础培养基、添加物和激素对其原球茎增殖及多糖含量的影响.结果表明:适合大苞鞘石斛原球茎悬浮培养的基本培养基为MS和B5培养基,增殖系数分别为4.31和4.03,多糖含量分别为7.08%和7.12%;添加物为香蕉+椰子汁时,增殖系数和多糖含量最高,分别为3.91和9.97%;适合原球茎悬浮培养的激素组合为0.5 mg/LNAA+0.2 mg/L6-BA.
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